Protein_Domain

Part:BBa_K3410002:Design

Designed by: Neele Kusch   Group: iGEM20_Bielefeld-CeBiTec   (2020-10-27)


Nanobody Estradiol


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The estradiol nanobody was grafted using an excel sheet and the AHo numbering scheme


Source

Acceptor: cAbBCII-10 [1]

Donor: 10G6D6 [2,3]

References

[1] C. Vincke, R. Loris, S. Muyldermans, and K. Conrath, Structure of CabBCII-10 nanobody, 2008.
[2] C. Monnet et al., Crystal Structure of Fab-Estradiol Complexes, 2002.
[3] C. Monnet et al., “Highly specific anti-estradiol antibodies: structural characterisation and binding diversity,” Journal of molecular biology, vol. 315, no. 4, pp. 699–712, 2002, doi: 10.1006/jmbi.2001.5284